Objective The aim of this work is to establish the expert consensus（hereinafter referred to as“consensus”） on clinical practice of injection safety in hemodialysis,to standardize the clinical practice of injection safety in hemodialysis and ensure patient safety during hemodialysis treatments. Methods Reviewing relevant literature and evidence-based nursing,summarizing the clinical work experience of care,we seeked for the best clinical evidence of injection safety. The consensus was performed by adjusting,modifying and perfecting each entry through three-round Delphi expert consultations and discussions. Results The consensus summarizes the latest evidence in 9 aspects,including environmental,personnel,syringes,safe administration of drugs,safe use of disinfectants,nursing practices on vascular access,specimen collection,medical waste and occupational safety,which covered all the elements and links in the hemodialysis therapy. In the three-round Delphi expert consultation and discussion,the positive coefficients were all 100% and the expert authority coefficients were all 0.928. The importance assignments were 4.38,4.45 and 4.68,respectively. The experts reached a fair degree of agreement. There were significant differences in experts’ coordination coefficients in the 3 rounds（P<0.05）. Conclusion The consensus shows certain scientific natrue,and it may provide evidence for injection safety in hemodialysis therapy in China.